Essential Reading

Methods

A very good introduction to subtomogram averaging in cryo-electron tomography can be found here: Turoňová & Wan. Focus Struct. Biol. 2024.

In addition, the following references would also be useful background for some of the software packages we will use:

• Dose weighting: Grant & Grigorieff. eLife. 2015.
• AreTomo: Zheng et al. J. Struct. Biol.: X. 2022.
• 3D-CTF Correction: Turoňová et al. J. Struct. Biol. 2017.

Application

A few recent implementations of the pipeline we’ll be using:

• "The palisade layer of the poxvirus core is composed of flexible A10 trimers" Liu et al. Nat. Struct. Mol. Biol. 2023.
• "In situ architecture of the ciliary base reveals the stepwise assembly of intraflagellar transport trains" van den Hoek et al. Science. 2022
• "Membrane-anchored HDCR nanowires drive hydrogen-powered CO2 fixation" Dietrich et al. Nature. 2022
• "Molecular-scale visualization of sarcomere contraction within native cardiomyocytes" Burbaum et al. Nat. Commun. 2021.
• "In situ cryo-electron tomography reveals gradient organization of ribosome biogenesis in intact nucleoli" Erdmann et al. Nat. Commun. 2021.